直立黄芪中产苦马豆素真菌的分离与鉴定

doi:10.11843/j.issn.0366-6964.2018.08.023

Abstract

Abstract:

The study was conducted to determine whether Astragalus adsurgens Pall is infected by the swainsonine-producing fungi, and the genetic evolution relationship between the Alternaria Section Undifilum species fungal endophytes from locoweeds and A. adsurgens Pall. Fungal isolates were isolated from the tissues of Astragalus adsurgens Pall. To screen the fungi that can produce swainsonine, the hyphal extract of each isolate was detected by means of the α-mannosidase Assay and ULPC-MS respectively. ITS, GPD, and KS genes from the swainsonine-producing fungi were amplified and sequenced for the analysis of homology and genetic evolution. The phylogenetic trees of fungi were built based on fungal ITS and GPD sequences. The taxon of the swainsonine-producing isolates from A. adsurgens Pall was determined according to morphology and the results of genetic evolution analysis. The present study showed that 43 isolates were cultured from the tissues of A. adsurgens Pall. The hyphae of thirteen isolates were confirmed containing swainonine. The results based on the analysis of homology and genetic evolution showed that swainonine-producing isolates were closely related to pathogenic fungus Alternaria gansuense from A. adsurgens Pall and the fungal endophytes Alternaria Section Undifilum sp. from locoweeds. This study determined the presence of swainonine-producing fungi inside A. adsurgens Pall. The isolates producing swainsonine are classified into Alternaria Section Undifilum sp and named Alternaria Section Undifilum gansuense based on the morphology and the results of genetic evolution analysis.

CLC Number:

S852.66

YU Yong-tao, LI Jin-rong, ZHAO Qing-mei, HE Sheng-hu, CHEN Juan, YANG Qi, MAO Yan-ni. Isolation and Identification of Swainsonine-producing Fungi from Astragalus adsurgens Pall[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1770-1780.

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Isolation and Identification of Swainsonine-producing Fungi from Astragalus adsurgens Pall

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